Background: Buffalo reactivity during milking affects milking procedures, milk Yield, and quality. Aims: This study evaluated the influence of milking reactivity on the Yield, composition, somatic cell count, pH, and electrical resistance of milk in Jaffarabadi buffaloes. Methods: A 1-4 point scale, based on leg movement, was used to assess the milking reactivity of buffaloes (n=40). Based on the milking reactivity score, animals were classified into four groups: reactivity score-1 (RS1), reactivity score-2 (RS2), reactivity score-3 (RS3), and reactivity score-4 (RS4). The influence of milking reactivity on Yield, composition, somatic cell count, pH, and electrical resistance of milk was observed. Results: Buffaloes with RS1 and RS2 produced significantly (P≤0.001) higher Daily milk Yield, 6% fat-corrected Yield, solid-corrected Yield, and energy-corrected Yield than the RS3+4 group. Milking reactivity score did not influence milk fat, protein, lactose, ash, solid-not-fat, total solids content, and the fat: protein ratio. However, Daily Yield of milk fat (P<0.001), protein (P=0.001), lactose (P=0.001), ash (P=0.002), solid-not-fat (P=0.001), and total solids (P<0.001) were significantly higher in buffaloes in the RS1 and RS2 groups than in the RS3+4 group. Milk somatic cell count and somatic cell score were not influenced by milking reactivity score (P>0.05). Milk density and pH did not differ significantly (P>0.05) between groups. However, the electrical resistance of milk in the RS1 group was significantly (P<0.05) higher than in the RS2 and RS3+4 groups. Conclusion: Milking reactivity influences Daily milk Yield, milk component Yield, and electrical resistance, but not milk composition in Jaffarabadi buffaloes.